ANTIBODY ARRAY ASSAY SERVICE

Send your samples to us. We’ll send results to you!

Send your samples to our lab for the complete antibody array assay service. Researchers can select from a wide range of antibody microarrays to study protein changes and phosphorylation events. Once we receive your samples, whether they are cells, tissues or serum, our experienced scientists will perform complete antibody array assays. This convenient assay service offers quick turnaround, accurate and reliable results, saving you time and resources!

SERVICES INCLUDED

  • Protein extraction and labeling
  • Assay condition optimization
  • Conjugation of biotinylated sample to to antibody array
  • Detection using Cy3-streptavidin
  • Array scanning
  • Image quantification and data analysis

RESULTS

  • Turnaround time: 5-10 business days for 2-5 samples
  • Assay data provided in Excel format
  • Assay protocol and data interpretation guidelines
  • Results are delivered to investigators by email
  • Sample Assay Results

ASSAY PRINCIPLE

1) Protein extracts are prepared with non-denaturing lysis buffer. Suitable Samples include cells, tissues, lysates, culture media, serum, FFPE

2) Proteins are labeled with biotin.

3) Biotinylated samples are incubated with antibody array.

4) Detection by dye conjugated streptavidin.

Antibody Array Assay Principle

SAMPLE PREPARATION AND SUBMISSION

Sample Requirements
  • Cells: 5 – 10 million
  • Tissues: > 75 mg
  • Serum: 5-10 uL
  • Lysates:
    • Protein amount: > 400 ug
    • Preferred concentration: > 2 ug/uL
    • Lysis buffer: mild, non-denaturing lysis buffer
Submission Process
  • Pack samples with sufficient dry ice.
  • Send by FedEx/UPS overnight or international priority service.
  • Forward submission and package tracking details to .
  • Forms required:
    • Sample Submission Form: WORD / PDF
    • Shipper’s Declaration (International only): WORD / PDF
User’s Guide
Assay Service Guide

ARRAY SELECTION

Phosphorylation Profiling Arrays

Array Name Catalog No. Price (Per Sample)
AKT/PKB Signaling Phospho ArraySEV03-PAB216$1,520
AMPK Signaling Phospho ArraySEV03-PAM174$1,470
Apoptosis Phospho ArraySEV03-PAP247$1,520
Cancer Signaling Phospho ArraySEV03-PCS248$1,520
Cell Cycle Control Phospho ArraySEV03-PCC238$1,520
Cell Cycle Phospho ArraySEV03-PCC076$1,240
Cell Signaling Phospho ArraySEV03-PCS300$1,730
CREB Phospho ArraySEV03-PCR174$1,470
Cytoskeleton Phospho ArraySEV03-PCP141$1,370
EGF Pathway Phospho ArraySEV03-PEG214$1,520
ErbB/HER Signaling Phospho ArraySEV03-PER239$1,520
Erk Signaling Phospho ArraySEV03-PEK208$1,520
GPCR Phospho ArraySEV03-PGP193$1,470
IGF-IR Signaling Phospho ArraySEV03-PIR245$1,520
Insulin Receptor Phospho ArraySEV03-PIG219$1,520
Jak/Stat Pathway Phospho ArraySEV03-PJS042$1,240
Jak/Stat II Phospho ArraySEV03-PJS202$1,470
MAPK Pathway Phospho ArraySEV03-PMK185$1,470
mTOR Phospho ArraySEV03-PMT138$1,370
Neuroscience Phospho ArraySEV03-PNS170$1,470
NF-kB Signaling Phospho ArraySEV03-PNK215$1,520
p53 Signaling Phospho ArraySEV03-PFT196$1,470
Phospho Explorer ArraySEV03-PEX100$2,310
T-Cell Receptor Signaling Phospho ArraySEV03-PTC188$1,520
TGF-beta Signaling Phospho ArraySEV03-PTG176$1,470
Tyrosine Kinase Adaptor Phospho ArraySEV03-PTK098$1,240
Tyrosine Phosphorylation ProArraySEV03-PST228$1,520
VEGF Pathway Phospho ArraySEV03-PVE185$1,470
Wnt Signaling Phospho ArraySEV03-PNT227$1,520

Total Protein Profiling Arrays

Array Name Catalog No. Price (Per Sample)
Apoptosis Antibody ArraySEV04-APP069$1,240
Cancer BioMarker Antibody ArraySEV07-SCB200$1,520
Cell Cycle Antibody ArraySEV04-ACC058$1,240
Cytokine Profiling Antibody ArraySEV07-SCK100$1,730
Explorer Antibody ArraySEV04-ASB600$1,730
Inflammation Antibody ArraySEV07-SMA090$1,240
Kinase Antibody ArraySEV05-AVK276$1,730
MAPK Signaling Antibody ArraySEV07-SMK060$1,240
Signal Transduction Antibody ArraySEV04-AST160$1,470
Signaling Explorer ArraySEV06-SET100$2,310

HOW TO ORDER

US Customers
International Customers
  • Contact us to obtain a proforma invoice.
  • Send payment by electronic bank transfer or by check.
  • Confirm receipt of payment with us.
  • Ship your samples to Full Moon BioSystems. Please refer to the Antibody Array Service Guide for sample preparation and shipping instructions.

QUESTIONS?

Please feel free to call 408.737.2875, email, or complete the form below if you have any questions regarding the assay service.

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