ANTIBODY ARRAYS FOR PROTEIN EXPRESSION

Each pack of arrays comes with two identical slides. Each slide can be used to run a single sample. You will need two slides (1 pack) to run a pair of samples, an untreated sample and a treatment sample.

In addition to the array slides, you will need the Antibody Array Assay Kit and dye-streptavidin that is compatible with your scanner. Please see the User’s Guide for requirements of dye-streptavidin.

Compatible samples include cells, tissues, serum, culture media, and FFPE. Generally, the recommended lysate concentration is 2 ug/uL or higher with 80 – 150 ug of protein for each array.

The antibody array platform is semi-quantitative. The data provides relative fold changes between an untreated sample and a treated sample.

A microarray scanner is needed to image the arrays. Here is a list of commonly used scanners. If you don’t access to a scanner, please consider our free array scanning service.

Certain gel imagers, such as, GE Healthcare’s Typhoon Imager and Li-Cor’s Odyssey system, may be used to image analysis. However, some of the systems may not be equipped with analysis module for microarrays. Please verify your system’s capability before using.

The software application that comes with your microarray scanner can be used for image quantification/data extraction. For example, GenePix® Pro is the acquisition and analysis software for GenePix® scanners.

To expedite spot finding, GAL files are provided free of charge and can be downloaded from here. Most modern analysis software programs are compatible with GAL files. If you don’t have access to a commercial software, please consider our image analysis service or open-source software, ImageJ, made available by NIH.

Our Complete Assay Service allows researchers to send samples to our lab in Sunnyvale, California. The assay will be conducted by our experienced scientists, and results will be delivered in electronic format by email. The turnaround time depends on the number of samples to be assayed. For 2-4 samples, the current timeline is 5-15 business days. For more details, please visit our complete assay service section.

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