T-Cell Receptor Phospho Antibody Array
The newly updated T-Cell Receptor Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein expression profiling. It is suitable for comparing protein expressions between non-treated and treated samples and identifying candidate biomarkers related to TCR signaling. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine, serine, and threonine phosphorylation at specific sites.
Key Features
- Site-specific phosphorylation profiling and screening with 213 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- 6 replicates/antibody
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for analyzing two samples (control vs. treated) |
| Featured Antibodies: | 213 site-specific and phospho-specific antibodies; 6 replicates per antibody |
| Reactivity: | Human: 99% | Mouse: 82% | Rat: 55% |
| Suitable Sample Type: | Cell lysate | Tissue lysate |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
Product Details
AKT(Ser473), AKT(Thr308), AKT(Tyr326), Calmodulin(Thr79/Ser81), CD127, CD134 (OX40), CD137, CD152, CD154, CD197 (CCR7), CD2, CD25, CD27, CD272, CD278 (ICOS), CD279, CD28, CD28(Tyr218), CD3, CD39, CD3Z(Tyr142), CD4, CD4(Ser433), CD45(Ser1007), CD45RA, CD45RO, CD5, CD62L, CD69, CD7, CD73, CD8, c-Jun(Ser243), c-Jun(Ser63), c-Jun(Ser73), c-Jun(Thr239), c-Jun(Thr91), c-Jun(Thr93), c-Jun(Tyr170), ERK1(Thr202), ERK1(Tyr204), ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177), Fos(Ser362), Fos(Thr232), FosB(Ser27), GAD1/GAD2, GAD67/GAD1, GADD153, GRB2(Ser159), IkB-alpha(Ser32/36), IkB-alpha(Tyr305), IkB-alpha(Tyr42), IkB-beta(Thr19), IkB-epsilon(Ser22), IKK alpha(Thr23), IKK alpha/beta(Ser180/181), IKK beta(Tyr188), IKK beta(Tyr199), IKK gamma(Ser31), IKK gamma(Ser85), IP3KA, IP3KC, JNK1/2/3(Thr183/Tyr185), LAT(Tyr171), LAT(Tyr191), LAT3, LCK(Ser59), LCK(Tyr192), LCK(Tyr393), LCK(Tyr504), MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4, MKK3(Ser189), MKK3/MAP2K3(Thr222), MKK6(Ser207), MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275), NFAT3, NFAT4(Ser165), NFkB-p100/p52(Ser865), NFkB-p100/p52(Ser869), NFkB-p105(Ser927), NFkB-p105/p50(Ser337), NFkB-p105/p50(Ser932), NFkB-p65(Ser536), NFkB-p65(Thr254), p38 MAPK(Thr180), p38 MAPK(Tyr182), p38 MAPK(Tyr322), Perforin, PI3K-p85-a(Tyr607), PI3K-p85-a/g(Tyr467/199), PKC pan activation sites, PKC theta(Ser676), PKC theta(Thr538), Rac1/cdc42(Ser71), Raf1(Ser259), Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341), Ras-GRF1(Ser916), Rel(Ser503), RelB(Ser552), SLP-76(Tyr128), VAV(Tyr174), VAV2(Tyr142), WASP(Tyr290), ZAP-70(Tyr292), ZAP-70(Tyr315), Zap-70(Tyr319), Zap-70(Tyr493)
The ELISA based Explorer Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
Ghosh D, Pham TD, Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells, Cell Rep. 2022 Jan 25;38(4):110200
Khameneh HJ, Leong KWK, The Inflammasome Adaptor ASC Intrinsically Limits CD4+ T-Cell Proliferation to Help Maintain Intestinal Homeostasis, Front Immunol. 2019; 10: 1566.
Services
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $250 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,520 per sample
