Signal Transduction Antibody Array
Signal Transduction Antibody Array is a high-throughput ELISA based antibody array, designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.
Key Features
- Qualitative/semi-quantitative protein expression profiling with 165 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for two samples (untreated vs. treated) |
| Featured Antibodies: | 165 antibodies; 6 replicates per antibody |
| Reactivity: | Human |
| Suitable Sample Type: | Cell lysate | Tissue lysate | Serum | Culture Media |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | Positive controls: beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
PRODUCT DETAILS
14.3.3 gamma, 14.3.3 Pan, 14-3-3 beta, Adenovirus, Adenovirus Fiber, Adenovirus Type 2 E1A, Adenovirus Type 5 E1A, ADP-ribosylation Factor, Alkaline Phosphatase, Alpha Fetoprotein, Alpha Lactalbumin, alpha-1-antichymotrypsin, alpha-1-antitrypsin, Amphiregulin, Amylin Peptide, Amyloid A, Amyloid A4 Protein Precursor, Amyloid Beta, Androgen Receptor, Bonzo/STRL33/TYMSTR, c-Abl, CD8, CDC25C, Chk1, c-JUN, CREB, CREB-Binding Protein, c-Src, DJ-1, DNA Ligase I, DNA Polymerase Beta, DNA Polymerase Gamma, DNA Primase(p49), DNA Primase(p58), DNA-PKcs, DP-2, DR5, Dysferlin, Dystrophin, Dystrophin, E2F-1, E2F-2, E2F-3, E2F-4, E2F-5, E3-binding protein, EGFR, EMA/CA15-3/MUC-1, Endostatin, Epithelial Membrane Antigen, Epithelial Specific Antigen, ER beta, ER Ca+2 ATPase2, ERCC1, ERK1, ERK2, Estriol, Estrogen Receptor, Ezrin/p81/80K/Cytovillin, Factor VIII Related Antigen, FADD, Fli-1, FLIP, FLT-1/VEGFR1, Flt-4, Fra2, FSH, FSH-b, Fyn, Gab-1, GCDFP-15, Granzyme B, Grb2, GRIP1, GSK-3, GSK3b, HDAC1, Heat Shock Factor 1, Heat Shock Factor 2, hsp27, hsp60, hsp70, 75/hsp75, hsp86, hsp84, Hepatic Nuclear Factor-3B, Hepatocyte, Hepatocyte Factor Homologue-4, Hepatocyte Growth Factor, HIF-1a, Histone H1, HPV 16, HPV 16-E7, IFN gamma, IgA, IGF-1R, IGF-I, IgM (m-Heavy Chain), IKKb, IL-2, IL-3, IL-30, IL-4, IL-5, IL-6, IL-10, IL-10R, IL17, Insulin, Insulin Receptor, Insulin Receptor Substrate-1, Int-2 Oncoprotein, Integrin beta5, Interferon-a(II), Interferon-g, IRAK, ITK, JKK1, Lck, Lck(p56lck), Leukotriene (C4,D4,E4), LewisA, LewisB, Maltose Binding Protein, MAP1B, MAP2a-b, MART-1, Mcl-1, Mek1, Mek2, Mek6, Mekk-1, Microphthalmia, Nck, NF kB/p50, NFkB/p65, NOS-i, NOS-u, P53, PAX-5, PDGF, PDGFR-a, PHAS-I, PHAS-II, Phospho-Ser/Thr/Tyr, Phosphotyrosine, Raf1, Raf-1(Phospho), Rhodopsin (Opsin), Ribonucleoprotein, SHP-1, SIM Ag, SIRP a1, Stat-1, Stat3, Stat5, Stat5a, Stat5b, Stat6, Superoxide Dismutase, Syk, TNFa, TTF-1
The ELISA based Signal Transduction Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
ADDITIONAL SERVICES
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $200 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,470 per sample
