Protein G Fluorescent Nanobeads

Protein G Fluorescent Nanobeads are highly specialized, versatile tools designed for the efficient capture and detection of immunoglobulins (IgG) from a wide range of species. These nanobeads are conjugated with Protein G, a bacterial cell wall protein that has a high affinity for the Fc region of IgG antibodies. Featuring a unique porous surface and internally covalently attached fluorescent dyes, these nanobeads offer exceptional sensitivity and reliability for use in immunoassays, cell sorting, and protein interaction studies.

Key Features

• High Affinity Protein G: The nanobeads are conjugated with Protein G, which specifically binds to the Fc region of IgG antibodies across multiple species. This high-affinity interaction ensures robust and selective capture of antibodies, making these beads ideal for sensitive assays.
• Porous Surface Design: The nanobeads feature a porous surface that enhances their binding capacity and interaction with target molecules, ensuring efficient capture and detection of IgG antibodies.
• Covalently Attached Dyes: The fluorescent dyes (FITC for green fluorescence and Cy5 for red fluorescence) are covalently bonded inside the nanobeads, ensuring long-term stability and resistance to photobleaching. This design protects the dye, reducing the risk of signal loss over time and enhancing the reliability of your results.
• Versatile Fluorescence: Available in FITC and Cy5, the beads offer flexibility in experimental design, allowing for multiplexing and the use of different fluorescence channels for diverse applications.

Applications

• Immunoprecipitation: Capture and isolate specific IgG antibodies or antibody-bound complexes from complex biological samples with high efficiency and low background.
• Flow Cytometry: Analyze and sort cells or particles based on IgG antibody binding, utilizing the bright and stable fluorescence from FITC or Cy5 for clear and precise detection.
• Fluorescence Microscopy: Achieve detailed visualization of IgG antibodies in cells or tissues, with the porous surface and internal dye attachment ensuring bright, stable, and consistent fluorescence.
• Protein Interaction Studies: Investigate protein-protein interactions by capturing IgG antibodies bound to their targets, using the strong fluorescent signals for easy detection and quantification.

Specifications

• • Protein G Conjugation: High-affinity binding to the Fc region of IgG antibodies
  • Material: Silica
  • Size: 400 – 500 um
  • Dyes:
    • FITC: Excitation: 488 nm; Emission: 520 nm
    • Cy5: Excitation: 646 nm; Emission: 670 nm
  • Stability: Stable for up to 12 months under proper storage conditions.

Storage Conditions

• • Store at 2-8°C, protected from light.
  • Do not freeze.
  • Ensure that the beads are well suspended before use.