Phospho Explorer Antibody Array

Phospho Explorer Antibody Array

Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples from human, mouse and rat. This array consists of 1318 antibodies related to multiple signaling pathways and biological processes. Each package contains two identical array slides for analyzing two samples, such as a control sample and a treatment sample.

$1,690.00

Cat #: PEX100

Phospho Explorer Antibody Array

Protein phosphorylation plays an important role in cell signaling and metabolism. Phospho Explorer Antibody Array serves as a high-throughput, ELISA based, phosphorylation assay for qualitative protein phosphorylation profiling. It is designed for comparing normal samples to treated or diseased samples and identifying candidate biomarkers. This phospho antibody array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine and serine/threonine phosphorylation at specific sites.

Key Features

  • Phosphorylation profiling with 1318 site-specific antibodies from over 30 signaling pathways
  • Antibodies covalently immobilized on 3D polymer coated glass slide
  • Fluorescent detection

 

Specifications

Product Size:2 array slides per package for analyzing two samples (untreated vs. treated)
Featured Antibodies:1318 site-specific and phospho-specific antibodies; 2 replicates per antibody
Reactivity:Human: 99% | Mouse: 92% | Rat: 66%
Suitable Sample Type:Cell lysate | Tissue lysate
Detection Method:Fluorescence | Compatible Scanners
Internal Controls:beta-actin | GAPDH | Negative controls
Slide Dimensions:76 x 25 x 1 mm
Storage Condition:4°C for 6 months

 

Product Details

14-3-3 theta/tau (Ser232), 14-3-3 zeta (Ser58), 14-3-3 zeta/delta (Ab-232), 14-3-3 zeta/delta (Thr232), 4E-BP1 (Ser65), 4E-BP1 (Thr36), 4E-BP1 (Thr45), 4E-BP1 (Thr70), Abl1 (Tyr204), Abl1 (Tyr412), ACC1 (Ser79), ACC1 (Ser80), ACK1 (Tyr284), AFX (Ser197), AKT (Ser473), AKT (Thr308), AKT (Tyr326), AKT1 (Ser124), AKT1 (Ser246), AKT1 (Thr450), AKT1 (Thr72), AKT1 (Tyr474), AKT2 (Ser474), ALK (Tyr1507), ALK (Tyr1604), AMPK1 (Thr174), Amyloid beta A4 (Thr743/668), Androgen Receptor (Ser213), Androgen Receptor (Ser650), A-RAF (Tyr301/302), Arrestin-1 (Ab-412), Arrestin-1 (Ser412), ASK1 (Ser83), ASK1 (Ser966), ATF-1 (Ser63), ATF2 (Ab-112/94), ATF2 (Ser112/94), ATF2 (Ser62/44), ATF2 (Thr69/51), ATF2 (Thr71/53), ATF2 (Thr73/55), ATF4 (Ser245), ATP1A1/Na+K+ ATPase1 (Ser23), ATPase (Ser16), ATP-Citrate Lyase (Ser454), ATRIP (Ser68/72), AurB (Thr232), AurB (Tyr12), Aurora Kinase (Thr288), AXL (Tyr691), and more.

The ELISA based Phospho Explorer Antibody Array platform involves four major steps:

  1. Protein extraction with non-denaturing lysis buffer
  2. Biotinylation of protein samples
  3. Incubation of labeled samples with antibody array
  4. Detection by dye conjugated streptavidin

Antibody Array - How It Works

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Services

If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.

Cost: Free

Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.

Cost: $350 per slide

Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.

Cost: $2,310 per sample

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