| Product Name | PDPK1 Rabbit mAb |
| Background | Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Function: Phosphorylates and activates not only PKB/AKT but also PKA, PKC-zeta, RPS6KA1, and RPS6KB1. May play a general role in signaling processes and development (by similarity). Isoform 3 is catalytically inactive. PTM: Phosphorylated on tyrosine and serine/threonine residues. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. Similarity: Belongs to the protein kinase superfamily, specifically the AGC Ser/Thr protein kinase family, PDK1 subfamily. Contains one PH domain and one protein kinase domain. Subcellular location: Membrane-associated after cell stimulation, leading to its translocation. Tyrosine phosphorylation appears to occur only at the plasma membrane. Subunit: Interacts with TUSC4. Tissue specificity: Appears to be expressed ubiquitously. |
| Reactivity | Human, Mouse, Rat |
| Source | Rabbit, IgG Kappa |
| Applications | WB; IHC; IF; IP; ELISA |
| Dilutions | WB: 1:2000-1:10000; IHC: 1:200-1:1000; IF: 1:200-1:1000; IP: 1:50-1:200; ELISA: 1:5000-1:20000 |
| Purification | Protein A |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage and Stability | -20°C/1 year |
| MW (kDa) | Calculated: 63; Observed: 58-68 |
| UniProt Accession # | O15530 |
| Protein Name | PDPK1 |
| Gene Name | PDPK1 |
| Gene ID | 5170 |
| Other Names | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
