p53 Signaling Phospho Antibody Array
p53 Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine phosphorylation and serine/threonine phosphorylation at specific sites.
Key Features
- Site-specific phosphorylation profiling and screening with 196 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for analyzing two samples (untreated vs. treated) |
| Featured Antibodies: | 196 site-specific and phospho-specific antibodies; 6 replicates per antibody |
| Reactivity: | Human: 100% | Mouse: 84% | Rat: 61% |
| Suitable Sample Type: | Cell lysate | Tissue lysate |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | Positive controls: beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
Product Details
ATM, ATRIP(Ser68/72), Bax, BCL-2(Ser70), BCL-2(Ser87), BCL-2(Thr56), BCL-2(Thr69), BRCA1(Ser1423), BRCA1(Ser1457), BRCA1(Ser1524), Breast tumor kinase, Casp3(Ser150), CDK2(Thr160), Chk1(Ser280), Chk1(Ser286), Chk1(Ser296), Chk1(Ser301), Chk1(Ser317), Chk1(Ser345), Chk2(Ser516), Chk2(Thr383), Chk2(Thr387), Chk2(Thr68), c-Raf(Ser296), c-Raf(Ser43), Cyclin D1(Thr286), Cyclin D3(Thr283), Cyclin E1(Thr395), Cyclin E1(Thr77), DNA-PK(Thr2638), DNA-PK(Thr2647), E2F1(Thr433), E2F2, E2F4, E2F6, ERK1/2, ERK3(Ser189), ERK8(Thr175/Tyr177), HDAC1(Ser421), HDAC10, HDAC2 (Ser394), HDAC3(Ser424), HDAC4(Ser632), HDAC5(Ser259), HDAC5(Ser498), HDAC6(Ser22), HDAC7, HDAC8(Ser39), HDAC9, JNK1/2/3(Thr183/Tyr185), JNKK, MAP3K1/MEKK1(Thr1381), MAPKAPK2(Ser272), MAPKAPK2(Thr222), MAPKAPK2(Thr334), MDM2(Ser166), MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MEKKK4, p21Cip1 (Ab-145), p21Cip1(Thr145), p300, p38 MAPK(Thr180), p38 MAPK(Tyr182), p38 MAPK(Tyr322), p44/42 MAPK(Thr202), p44/42 MAPK(Tyr204), p53(Ser15), p53(Ser20), p53(Ser315), p53(Ser33), p53(Ser366), p53(Ser37), p53(Ser378), p53(Ser392), p53(Ser46), p53(Ser6), p53(Ser9), p53(Thr18), p53(Thr81), PIAS1, PIAS2, PIAS3, PIAS4, PKC delta(Ser645), PKC delta(Thr505), PKC delta(Tyr313), PKC delta(Tyr52), PKC delta(Tyr64), Raf1(Ser621), Raf1(Ser259), Raf1 (Ser338), Raf1(Tyr341), Rb(Ser608), Rb(Ser780), Rb(Ser795), Rb(Ser807), Rb(Ser811), Rb(Thr821)
The ELISA based p53 Signaling Phospho Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
Mazer J, Rosado A, The long non-coding RNA GAS5 differentially regulates cell cycle arrest and apoptosis through activation of BRCA1 and p53 in human neuroblastoma, Oncotarget 2017; 8(4):6589-6607
Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. You will receive raw images in tiff format.
Cost: Free of Charge
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $200 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,470 per sample
