MAP Kinase (MAPK) Signaling Antibody Array
The mitogen-activated protein kinase (MAPK) Antibody Array is an ELISA based antibody array for studying MAP kinase signaling cascades. It is designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.
Key Features
- Qualitative/semi-quantitative protein expression profiling with 60 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for two samples (untreated vs. treated) |
| Featured Antibodies: | 63 antibodies; 6 replicates per antibody |
| Reactivity: | Human |
| Suitable Sample Type: | Cell lysate | Tissue lysate | Serum | Culture Media |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | Positive controls: beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
PRODUCT DETAILS
AKT1, AKT2, Akt3, A-RAF, ASK1 (MAP3K5), ASK2 (MAP3K6), ASK3 (MAP3K15), BRAF, c-RAF, CREB, Elk1, “ERK1 (MAPK3, p44-MAPK)”, “ERK2 (MAPK1, p42-MAPK)”, ERK3 (MAPK6), “ERK4 (MAPK4, p63MAPK)”, ERK7 (MAPK15), GSK3 alpha, GSK3 beta, HGK (MAP4K4), HSP27, JNK1 (MAPK8), JNK2 (MAPK9), JNK3 (MAPK10), KHS1 (MAP4K5), KHS2 (MAP4K3), LAMTOR3 (MAP2K1IP1), MAP2K4, MEK1 (MAP2K1), MEK2 (MAP2K2), MEK3 (MAP2K3), MEK5 (MAP2K5), MEK6 (MAP2K6), MEKK1 (MAP3K1), MEKK3 (MAP3K3), MEKK4 (MAP3K4), MINK (MAP4K6), MK2 (MAPKAPK2), MK3 (MAPKAPK3), MLK (MAP3K13), MLK1 (MAP3K9), MLK2 (MAP3K10), MLK3 (MAP3K11), MLK4 (MAP3KL4), MSK1 (RPS6KA5), mTOR, NF-kB p65, p38A (MAPK14), p38B (MAPK11), p38D (MAPK13), p38G (MAPK12), p44/42 MAPK, p53, p70S6K (RPS6KB1), p70S6K2 (RPS6KB2), PRAK (MAPKAPK5), RSK1 (RPS6KA1), RSK2 (RPS6KA3), RSK3 (RPS6KA2), RSK4 (RPS6KA6), RSKL1 (RPS6KC1), STAT1, TAB1 (MAP3K7IP1), TAK1 (MAP3K7)
The ELISA based MAPK Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
Don’t have access to a compatible microarray scanner? No problem! Simply send the finished array slides to our lab for scanning. You will receive raw images in tiff format.
Cost: Free of Charge
ADDITIONAL SERVICES
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $150 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,240 per sample
