Kinase Antibody Array
Kinase Antibody Array is a high-throughput ELISA based antibody array for qualitative/semi-quantitative protein expression profiling. This array includes antibodies from the kinase families AGC, CAMK, CK1, CMGC, STE, TK, and TKL. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.
Key Features
- Qualitative/semi-quantitative protein expression profiling with 276 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for two samples (untreated vs. treated) |
| Featured Antibodies: | 276 antibodies; 6 replicates per antibody |
| Reactivity: | Human |
| Suitable Sample Type: | Cell lysate | Tissue lysate | Serum | Culture Media |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | Positive controls: beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
PRODUCT DETAILS
ABL2, ACTR1B, ACVR1B, AHSA1, AKT1, AKT2, ANK1, ANP32A, ARG2, ATM, AXL, BCR, BMPR1A, BMPR1B, BMPR2, BRAF, BUB1, BUB1B, CAMK1, CAMK1D, CAMK1G, CAMK2A, CAMK2B, CAMK2D, CAMK4, CAMKK1, CAMKK2, CAMKV, CDC2, CDC25A, CDC25B, CDC25C, CDC2L2, CDK2, CDK3, CDK4, CDK5, CDK5R1, CDK5RAP3, CDK6, CDK7, CDK8, CDK9, CDKL1, CDKL2, CDKL3, CDKL4, CDKL5, CHEK1, CHEK2, CSF1, CSF1R, CSF2, CSF2RA, CSNK1D, CSNK1E, CSNK1G1, CSNK1G2, CSNK2A1, CSNK2A2, DMPK, DYRK1A, DYRK1B, DYRK2, DYRK3, DYRK4, EFNA1, EFNA3, EFNA5, EGFR, EIF2AK2, EPB41, EPB41L1, EPB41L3, EPB42, EPDR1, EPHA2, EPHA4, EPHA5, EPHA6, EPHA7, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6, EPHX1, EPM2A, ERBB2, ERBB2IP, ERBB3, FES, FGFR1, FGFR1OP, FGFR1OP2, FGFR2, FGFR4, FGFRL1, FGR, FLT1, FLT3, FLT3LG, FLT4, FRAP1, FUSIP1, FYN, and more
The ELISA based Kinase Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
Rahal OM, Wolfe AR, Blocking Interleukin (IL)4- and IL13-Mediated Phosphorylation of STAT6 (Tyr641) Decreases M2 Polarization of Macrophages and Protects Against Macrophage-Mediated Radioresistance of Inflammatory Breast Cancer, Int J Radiat Oncol Biol Phys. 2018 Mar 15;100(4):1034-1043
ADDITIONAL SERVICES
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $300 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,730 per sample
