ERK Signaling Phospho Antibody Array
ERK Signaling Phospho Antibody Array is a high-throughput ELISA based antibody array for qualitative protein phosphorylation profiling. It is suitable for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers. This array features site-specific and phospho-specific antibodies, allowing researchers to study tyrosine, serine, and threonine phosphorylation at specific sites.
Key Features
- Site-specific phosphorylation profiling and screening with 227 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for analyzing two samples (untreated vs. treated) |
| Featured Antibodies: | 227 site-specific and phospho-specific antibodies; 6 replicates per antibody |
| Reactivity: | Human: 100% | Mouse: 96% | Rat: 83% |
| Suitable Sample Type: | Cell lysate | Tissue lysate |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
PRODUCT DETAILS
A-Raf(Tyr301/302), Arrestin-1(Ser412), BAD (Ab-91/128), BAD(Ser112), BAD(Ser134), BAD(Ser136), BAD(Ser155), BAD(Ser91/128), B-Raf(Ser446), B-Raf(Ser601), B-Raf(Thr598), CaMK1-alpha(Thr177), CaMK1-beta, CaMK2(Thr286), CaMK2-beta/gamma/delta(Thr287), CaMK4(Thr196/200), c-Raf(Ser296), c-Raf(Ser43), CREB(Ser121), CREB(Ser129), CREB(Ser133), CREB(Ser142), CREB(Thr100), DYN1(Ser774), Elk1(Ser383), Elk1(Ser389), Elk1(Thr417), ERK3(Ser189), ERK8(Thr175/Tyr177), Fos(Thr232), FosB(Ser27), GRB2(Ser159), GTPase activating protein (Phopspho-Ser387), Histone H3.1(Ser10), IkB-alpha(Ser32/36), IkB-alpha(Tyr42), IKK-alpha(Thr23), IKK-alpha/beta(Ser180/181), IP3KA, IP3KC, IP6K2, IP6K3, MEK1(Ser217), MEK1(Ser221), MEK1(Ser298), MEK1(Thr286), MEK1(Thr291), MEK2(Thr394), MEKKK1, MKK3(Ser189), MKK3/MAP2K3(Thr222), MKK6(Ser207), MKK7/MAP2K7(Ser271), MKK7/MAP2K7(Thr275), Mnk1(Thr385), MSK1(Ser212), MSK1(Ser360), MSK1(Ser376), MSK1(Thr581), MSK2(Thr568), Myc(Ser373), Myc(Ser62), Myc(Thr358), Myc(Thr58), p44/42 MAP Kinase(Thr202), p44/42 MAP Kinase(Thr202/Tyr204), p44/42 MAP Kinase(Tyr204), PAK1(Ser204), PAK1(Thr212), PAK1/2(Ser199), PAK1/2/3(Ser141), PAK1/2/3(Thr423/402/421), PAK2(Ser192), PAK3(Ser154), PI3-kinase p85-alpha(Tyr607), PI3-kinase p85-subunit alpha/gamma(Tyr467/Tyr199), PKA CAT(Thr197), PKC alpha (Tyr657), PKC alpha/beta II(Thr638), PKC beta(Ser661), PKC delta(Ser645), PKC delta(Thr505), PKC epsilon(Ser729), PKC theta(Ser676), PKC theta(Thr538), PKC zeta(Thr410), PKC zeta(Thr560), PKR(Thr446), PKR(Thr451), PLC-beta(Ser1105), PLC-beta3(Ser537), PLCG1(Tyr771), PLCG1(Tyr783), PLCG2(Tyr1217), PLCG2(Tyr753), PP2A-a(Tyr307), Pyk2(Tyr402), Pyk2(Tyr579), Pyk2(Tyr580), Pyk2(Tyr881), Rac1/cdc42(Ser71), Raf1(Ser259), Raf1(Ser338), Raf1(Ser621), Raf1(Tyr341), RapGEF1(Tyr504), RAS (p21 H&K), RASE, RASF4, Ras-GRF1(Ser916), Rho/Rac guanine nucleotide exchange factor 2(Ser885), RSK1/2/3/4(Ser221/227/218/232), Src(Ser75), Src(Tyr418), Src(Tyr529), SRF(Ser77), SRF(Ser99), STAT1(Ser727), STAT1(Tyr701), STAT3(Ser727), STAT3(Tyr705)
The ELISA based ERK Signaling Phospho Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
Antibody List
GAL File
Frequently Asked Questions
Hu L, Li L, Baicalin Inhibits Airway Smooth Muscle Cells Proliferation through the RAS Signaling Pathway in Murine Asthmatic Airway Remodeling Model, Oxidative Medicine and Cellular Longevity, vol. 2023, Article ID 4144138, 12 pages, 2023. https://doi.org/10.1155/2023/4144138
Lutz AK, Arevalo AP, SHANK2 Mutations Result in Dysregulation of the ERK1/2 Pathway in Human Induced Pluripotent Stem Cells-Derived Neurons and Shank2(−/−) Mice, Front. Mol. Neurosci., 26 November 2021, https://doi.org/10.3389/fnmol.2021.773571
Sebastian S, Weinstein L, Slowing heart rate protects against pathological cardiac hypertrophy, Function. 2022: zqac055
Urena F, Ma C, T cell activation decreases microRNA-15a/16 levels to promote MEK1-ERK1/2-Elk1 signaling and promote proliferative capacity, J Biol Chem 2022. doi: https://doi.org/10.1016/j.jbc.2022.101634
Zhang Z, Yan C, Potential biological functions of microvesicles derived from adenoid cystic carcinoma, Oncology letter, 2018 May 1;15(5):7900-8
ADDITIONAL SERVICES
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $250 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,520 per sample
