Cytokine Profiling Antibody Array
Cytokine Profiling Antibody Array is a high-throughput ELISA based antibody array for protein expression profiling of cytokines, chemokines and related biomarkers. The array is designed for comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.
Key Features
- 310 cytokine related antibodies; 6 replicates per antibody
- Qualitative/semi-quantitative protein expression profiling of cytokines and related biomarkers
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for two samples (untreated vs. treated) |
| Featured Antibodies: | 310 cytokine related antibodies; 6 replicates per antibody |
| Reactivity: | Human |
| Suitable Sample Type: | Cell lysate | Tissue lysate | Serum | Culture Media |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | Positive controls: beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Spot diameter: | 260 - 280 um |
| Storage Condition: | 4°C for 6 months |
Product Details
4-1BB Receptor, 4-1BBL, 4E-BP1, Adipolean Variant, Adiponectin, AFP, AITRL, AKT1, AKT2, ALCAM, alpha hCG, Androgen receptor, Angiopoietin-1, Angiopoietin-2, APC, ApoE3, Apolipoprotein F (APOF), Apolipoprotein L1 (APOL1), Apolipoprotein L2 (APOL2), APRIL, Artemin, AXL, BAFF, BCA-1, BCL-10, BD-1, BD-2, BD-3, BD-4, BDNF, beta hCG, Beta-2-Microglobulin, Betacellulin, BLK, BMP-2, BMP-4, BMP-7/OP-1, BRAK, CA125, CA15-3, CA19-9, Cadherin-pan, Cardiotrophin-1, Catenin-beta 1, Catenin-a1, Catenin-γ, CD14, CD40 , CD44, CEA, CIB1, C-Kit, CNTF, C-reactive Protein (CRP), CTACK, CTGF, CTGFL/WISP-2, CXCL16, E-cadherin, EGF, EGFR, EGR1, EG-VEGF, EMAP-II, ENA-78, Endostatin, Eotaxin, Eotaxin-2, Eotaxin-3, EPCAM, ERCC6, E-Selectin, Exodus-2, FAK, FAS, FER, Ferritin, FGF-10, FGF-16, FGF-17, FGF-4, FGF-5, FGF-acidic, FGF-basic, FGFR1 Oncogene Partner, FGFR2, FGFR3, FLI1, Flt-1, Flt3-Ligand, Follistatin, Fractalkine, Free PSA, FSH, gAcrp30/Adipolean, Galectin-1, Galectin-3, GATA1, GATA3, GCP-2, G-CSF, GDF-3, GDNF, GM-CSF, and more
The ELISA based Cytokine Profiling Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
Bae YU, You JH, Association of Protein Z with Prediabetes and Type 2 Diabetes, Endocrinol Metab (Seoul). 2021 Jun 2. doi: 10.3803/EnM.2021.962
Heo SK, Noh EK, The soluble VCAM-1 level is a potential biomarker predicting severe acute graft versus host disease after allogeneic hematopoietic cell transplantation, BMC Cancer. 2022; 22: 997
Hwang HJ, Jung SH, Identification of novel therapeutic targets in the secretome of ionizing radiation‑induced senescent tumor cells, Oncol Rep, 2016 35(2):841-850
Kang SH, Oh SY. Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p. Theranostics. 2024 Jan 1;14(2):460-479. doi: 10.7150/thno.87329
Luo MXM, Wong SH, Autophagy Mediates HBx-Induced Nuclear Factor-κB Activation and Release of IL-6, IL-8 and CXCL2 in Hepatocytes, Journal of Cellular Physiology, 2015 Oct;230(10):2382-9
Shin DH, Jo JY, Midkine Is a Potential Therapeutic Target of Tumorigenesis, Angiogenesis, and Metastasis in Non-Small Cell Lung Cancer, Cancer (Basel). 2020 Aug 24;12(9):E2402. doi: 10.3390/cancers12092402
Shin E, Kim D, LDR-adapted liver-derived cytokines have potential to induce atherosclerosis, Int J Radiat Biol. 2022. DOI: 10.1080/09553002.2023.2145028
Kim DH, Cho HJ, Transplantation of PSA-NCAM-Positive Neural Precursors from Human Embryonic Stem Cells Promotes Functional Recovery in an Animal Model of Spinal Cord Injury, Tissue Eng Regen Med. 2022 Aug 29. doi: 10.1007/s13770-022-00483-z
Services
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $300 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,730 per sample
