Apoptosis Antibody Array
Apoptosis Antibody Array is a high-throughput ELISA based antibody array. It is designed for qualitative/semi-quantitative protein expression profiling, comparing normal samples to treated or diseased samples, and identifying candidate biomarkers.
Key Features
- Qualitative/semi-quantitative protein expression profiling with 73 antibodies
- Antibodies covalently immobilized on 3D polymer coated glass slide
- Fluorescent detection
Specifications
| Product Size: | 2 array slides per package for two samples (untreated vs. treated) |
| Featured Antibodies: | 73 antibodies; 6 replicates per antibody |
| Reactivity: | Human |
| Suitable Sample Type: | Cell lysate | Tissue lysate | Serum | Culture Media |
| Detection Method: | Fluorescence | Compatible Scanners |
| Internal Controls: | Positive controls: beta-actin | GAPDH | Negative controls |
| Slide dimensions: | 76 x 25 x 1 mm |
| Storage Condition: | 4°C for 6 months |
PRODUCT DETAILS
14.3.3 Pan, Acinus, AIF, Alpha-1-antichymotrypsin, alpha-1-antitrypsin, ARC, Ask1, ATM, BAG-1, Bak, Bax, Bcl10, bcl-2a, Bcl-6, bcl-X, bcl-XL, Bim, Caspase 1, Caspase 2, Caspase 3, Caspase 5, Caspase 6, Caspase 7, Caspase 8, Cathepsin D, CD137, CD14, CD95, CDK2, CDK4, CDK5, c-fos, CIDE-A, CIDE-B, c-jun, CREB, CREB-Binding Protein, Cytochrome c, D4-GDI, Daxx, DcR1, DcR2, DFF40, DFF45, DR3, DR5, ERK1, ERK2, FADD, Fas-ligand, FLIP, Granzyme B, hsp70, I-FLICE, IGF-1R, IGF-I, IKKb, JKK1, MADD, Mcl-1, Mekk-1, NFkB/p50, NFkB/p65, p53, PARP, PARP, Prohibitin, RAIDD, SODD, Survivin, TNF alpha, TRADD
The ELISA based Apoptosis Antibody Array platform involves four major steps:
- Protein extraction with non-denaturing lysis buffer
- Biotinylation of protein samples
- Incubation of labeled samples with antibody array
- Detection by dye conjugated streptavidin
GAL File (To download, right click on the file name, then choose “Save target as”)
Frequently Asked Questions
Carriere PP, Kapur N, Cinnamtannin B-1 inhibits cell survival molecules and induces apoptosis in colon cancer, Inl J Oncol, 2018 53(4):1442-1454
Hu S, Han R, The long noncoding RNA LOC105374325 causes podocyte injury in individuals with focal segmental glomerulosclerosis, J Biol Chem, 2018 293(52):20227-20239
Lee, SY, Debnath T, Anti-cancer effect and apoptosis induction of cordycepin through DR3 pathway in the human colonic cancer cell HT-29, Food and Chem Toxicol. 2013, 60:439-447
Molnar E: Applications of Protein and Small Molecule Microarrays, Acta Biologica Szegediensis 2008, 52(2): 333-356
Saunders I, Mir H, Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways, Cancer Cell International, 2019 19:98
Zacarias O, Clement CC, Mitomycin C and Its Analog Trigger Cytotoxicity in MCF-7 and K562 cancer cells through the regulation of RAS and MAPK/ERK pathways, Chem Biol Interact. 2024 Apr 18:111007. doi: 10.1016/j.cbi.2024.111007.
ADDITIONAL SERVICES
If you don’t have access to a microarray, send the finished arrays to our lab for scanning. Raw scan images are delivered in tiff format.
Cost: Free
Array Image Quantification and Analysis Service includes data extraction, data organization and analysis of the array images obtained through our array scanning service.
Cost: $150 per slide
Complete Antibody Array Assay Service allows investigators to send research samples to our laboratory for analysis. There is no need to purchase the arrays and reagents and running the assays yourself. Simply select the array of your choice, and then send off the samples to our lab. This convenient hands-off approach offers quick turnaround and reliable results, saving you valuable time and resources. All assays will be performed by our highly trained scientists at our headquarter in Sunnyvale, California. Results are delivered by email in 1-3 weeks.
Cost: $1,240 per sample
