| Product Name | 53BP1 Rabbit mAb |
| Background | Function: May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. PTM: Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. Similarity: Contains 2 BRCT domains. Subcellular Location: Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double-strand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double-strand breaks. Subunit: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX, and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3. |
| Reactivity | Human |
| Source | Rabbit, IgG Kappa |
| Applications | WB; IF; IP; ELISA |
| Dilutions | WB: 1:2000-1:10000; IHC: /; IF: 1:200-1:1000; IP: 1:50-1:200; ELISA: 1:5000-1:20000 |
| Purification | Protein A |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage and Stability | -20°C/1 year |
| MW (kDa) | Calculated: 214; Observed: 450 |
| UniProt Accession # | Q12888 |
| Protein Name | 53BP1 |
| Gene Name | TP53BP1 |
| Gene ID | 7158 |
| Other Names | TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1 |